Kusagwiritsa ntchito ufa wa nsomba m'malo mwa ufa wa soya (SBM) ngati njira yokhazikika komanso yachuma kwafufuzidwa m'mitundu yambiri ya nsomba zomwe zimagulitsidwa, kuphatikizapo nsomba zamadzi oyera (rainbow trout).Oncorhynchus mykissKomabe, soya ndi zinthu zina zochokera ku zomera zimakhala ndi saponins wambiri ndi zinthu zina zotsutsana ndi zakudya zomwe zimayambitsa matenda a subacute enteritis m'matumbo akutali mwa nsomba zambirizi. Matendawa amadziwika ndi kuwonjezeka kwa kulowa m'mimba, kutupa, komanso kusintha kwa mawonekedwe a thupi zomwe zimapangitsa kuti chakudya chisamagwire bwino ntchito komanso kukula kwake kusokonezeke.

Mu nsomba zamtundu wa trout, kuphatikizapo SBM, zakudya zopitirira 20% zawonetsedwa kuti zimayambitsa matenda a soya, zomwe zimapangitsa kuti pakhale kuchuluka kwa chakudya chomwe chingalowe m'malo mwa zakudya zokhazikika za m'madzi. Kafukufuku wakale wafufuza njira zingapo zolimbana ndi matenda a enteritis, kuphatikizapo kusintha kwa microbiome ya m'matumbo, kukonza zosakaniza kuti muchotse zinthu zotsutsana ndi zakudya, komanso zowonjezera zotsutsana ndi ma antioxidants ndi ma probiotic. Njira imodzi yomwe sinafufuzidwe ndikuphatikiza trimethylamine oxide (TMAO) m'zakudya za m'madzi. TMAO ndi cytoprotective yodziwika bwino, yomwe imasonkhanitsidwa m'mitundu yambiri ngati protein ndi membrane stabilizer. Pano, tikuyesa kuthekera kwa TMAO kukulitsa kukhazikika kwa enterocyte ndikuletsa chizindikiro cha HSP70 chotupa motero kuthana ndi matenda a enteritis omwe amabwera chifukwa cha soya ndikupangitsa kuti chakudya chikhale bwino, kusunga ndi kukula kwa nsomba zamtundu wa trout zamadzi oyera. Kuphatikiza apo, tikuyang'ana ngati nsomba zam'madzi zosungunuka, zomwe ndi gwero lolemera la TMAO, zingagwiritsidwe ntchito ngati njira yothandiza pazachuma yoperekera izi zowonjezera, zomwe zimathandiza kuti zigwiritsidwe ntchito pamlingo wamalonda.

Nsomba zamtundu wa rainbow trout zolimidwa (Troutlodge Inc.) zinasungidwa pa kulemera koyambirira kwa 40 g ndi n = 15 pa thanki iliyonse m'matangi ochiritsira atatu. Matangi anapatsidwa chakudya chimodzi mwa zisanu ndi chimodzi chokonzedwa pamaziko a michere yomwe imagayidwa yomwe imapereka 40% ya mapuloteni ogayidwa, 15% ya mafuta osakonzedwa, komanso kukwaniritsa kuchuluka kwa amino acid koyenera. Zakudya zinaphatikizapo chakudya cha nsomba cholamulidwa ndi 40 (% ya zakudya zouma), SBM 40, SBM 40 + TMAO 3 g kg.^-1, SBM 40 + TMAO 10 g kg^-1, SBM 40 + TMAO 30 g kg^-1, ndi SBM 40 + 10% ya nsomba zosungunuka. Matanki anadyetsedwa kawiri patsiku kuti akhute kwa milungu 12 ndipo kusanthula kwa ndowe, pafupi, histological ndi molecular kunachitika.

Zotsatira za kafukufukuyu zidzakambidwa komanso kufunika kophatikiza TMAO kuti zithandize kugwiritsa ntchito kwambiri soya wa ku US mu nsomba za salmonid.