Pofuna kuwunika momwe kuwonjezera triglyceride mu zakudya kumakhudzira kupanga mapuloteni a tizilombo tating'onoting'ono ta m'mimba mwa nkhuku ndi momwe zimakhalira pakuphika kwa nkhosa zazikazi zazikulu, mayeso awiri adachitika mu vitro ndi mu vivo.

Kuyesa mu vitro: Zakudya zoyambira (zochokera ku zinthu zouma) zokhala ndi triglyceride yambiri ya 0, 2, 4, 6 ndi 8g / kg zinagwiritsidwa ntchito ngati gawo lapansi, ndipo madzi a rumen a nkhosa zazikazi zazing'ono za Adult Small Tailed anawonjezedwa, ndikusungidwa pa kutentha kwa 39 ℃ kwa maola 48 mu vitro.

Kuyesa kwa m'thupi: Nkhosa zazikazi 45 zazikulu zinagawidwa mwachisawawa m'magulu 5 malinga ndi kulemera kwawo koyambirira (55 ± 5 kg).Glyceryl tributylateZakudya zokwana 0, 2, 4, 6 ndi 8 g/kg (zochokera ku zinthu zouma) zinawonjezeredwa ku zakudya zoyambira, ndipo madzi a m'mimba ndi mkodzo zinasonkhanitsidwa kwa masiku 18.

Zotsatira za Mayeso

1). Zotsatira pa pH ndi kuchuluka kwa mafuta acid m'thupi

Zotsatira zake zasonyeza kuti pH ya malo osungiramo zinthu inachepa molunjika ndipo kuchuluka kwa total volatile fatty acids (TVFA), acetic acid, butyric acid ndi branched chain volatile fatty acids (BCVFA) kunawonjezeka molunjika pamenetributyl glycerideadawonjezedwa ku substrate. Zotsatira za mayeso a in vivo zidawonetsa kuti kudya kwa dry matter (DMI) ndi pH kunachepa, ndipo kuchuluka kwa TVFA, acetic acid, propionic acid, butyric acid ndi BCVFA kunawonjezeka motsatizana ndi kuwonjezera kwatributyl glycerideZotsatira za mayeso a in vivo zinasonyeza kuti kudya kwa dry matter (DMI) ndi pH kunachepa, ndipo kuchuluka kwa TVFA, acetic acid, propionic acid, butyric acid ndi BCVFA kunawonjezeka motsatizana ndi kuwonjezera kwa tributyl glyceride.

Zotsatira za mayeso a in vivo zinasonyeza kuti kudya kwa dry matter (DMI) ndi pH kunachepa, ndipo kuchuluka kwa TVFA, acetic acid, propionic acid, butyric acid ndi BCVFA kunawonjezeka motsatizana ndi kuwonjezera kwatributyl glyceride.

2). Kuonjezera kuchuluka kwa zakudya zomwe zimawonongeka

Kuchuluka kwa kuwonongeka kwa DM, CP, NDF ndi ADF kunawonjezeka motsatizana pamenetributyl glycerideidawonjezedwa ku substrate mu vitro.

3). Kupititsa patsogolo ntchito ya enzyme yowononga maselo a cellulose

Kuwonjezera kwatributyrinKuyesa kwa mu vitro kunawonjezera ntchito za xylanase, carboxymethyl cellulase ndi microcrystalline cellulase. Kuyesa kwa mu vivo kunawonetsa kuti triglyceride imawonjezera ntchito za xylanase ndi carboxymethyl cellulase.

4). Kupititsa patsogolo kupanga mapuloteni a tizilombo toyambitsa matenda

Mayeso a mu vivo adawonetsa kutitributyrinKuchuluka kwa allantoin tsiku lililonse, uric acid ndi purine ya microbial yomwe imayamwa mu mkodzo kumawonjezeka, komanso kuonjezera kupanga kwa nayitrogeni ya microbial ya rumen.

Mapeto

Tributyrinkunathandiza kuti mapuloteni a rumen microbial apangidwe, kuchuluka kwa mafuta acids ofooka komanso ntchito ya ma enzymes owononga cellulose, komanso kunalimbikitsa kuwonongeka ndi kugwiritsa ntchito zinthu zouma, mapuloteni osaphika, ulusi wothira detergent wosalowerera komanso ulusi wothira acid mu zakudya.

Zimasonyeza kuti triglyceride imakhudza bwino kukolola ndi kuwiritsa kwa mapuloteni a tizilombo ta m'mimba, ndipo ikhoza kukhala ndi zotsatira zabwino pa ntchito yobereka ya nkhosa zazikulu.