High mphamvu zokopaDMPTndiChithunzi cha DMTndi zatsopano komanso zokopa za nyama zam'madzi. Mu phunziro ili, zokopa zamphamvu kwambiriDMPTndiChithunzi cha DMTadawonjezeredwa ku chakudya cha carp kuti afufuze zotsatira za zokopa ziwiri pa kudyetsa carp ndi kulimbikitsa kukula. Zotsatira zinawonetsa kuti kuwonjezeredwa kwa zokopa zamphamvu kwambiriDMPTndiChithunzi cha DMTku chakudya kwambiri kuchuluka kuluma pafupipafupi nsomba experimental ndipo anali ndi kudyetsa kwenikweni; Pa nthawi yomweyi, kuwonjezeredwa kwa magulu osiyanasiyana a zokopa zamphamvu kwambiriDMPTndiChithunzi cha DMTku chakudya chinawonjezeka kwambiri kuchuluka kwa kulemera, kukula kwake, ndi kupulumuka kwa nsomba zoyesera, pamene chiwerengero cha chakudya chinachepa kwambiri. Zotsatira za kafukufuku zimasonyezanso kutiDMPTimakhala ndi mphamvu yochulukirapo pakukopa ndi kulimbikitsa kukula kwa carp poyerekeza ndiChithunzi cha DMT.

Zakudya zopatsa nyama zam'madzi ndizowonjezera zopanda thanzi. Kuwonjezera zinthu zokopa nsomba kuti zidyetse nsomba kungathandize kuti zidyetse, kuwonjezera zakudya zomwe zimadya, kuchepetsa zakudya zotsalira m'madzi, ndipo motero kuchepetsa kuipitsa m'madzi a m'madzi.DMPTndiChithunzi cha DMTndi zinthu zogwira ntchito zomwe zimapezeka kwambiri m'zamoyo zam'madzi, zomwe zimagwira ntchito ngati othandizira a methyl komanso owongolera osmotic pressure. Amakhalanso ndi kadyedwe kofunikira komanso kakulidwe kake ka nyama zam'madzi.

Atachita maphunziro oyenera pa nyama zam'madzi monga crucian carp, red snapper, goldfish, ndi shrimp zamawanga, ofufuza a ku Japan anapeza kuti.DMPTndiChithunzi cha DMTkukhala ndi zotsatira zabwino zokopa pamadzi amchere ndi nsomba zam'madzi, nkhanu, ndi nkhono. Kuonjezera kutsika kochepa kwa zokopa zamphamvu kwambiriDMPTndiChithunzi cha DMTmu chakudya akhoza kwambiri imathandizira kudyetsa ndi kukula zosiyanasiyana madzi abwino ndi nsomba za m'madzi. Pakuyesa uku, zokopa zamphamvu kwambiriDMPTndiChithunzi cha DMTadawonjezeredwa ku chakudya cha carp kuti aphunzire zotsatira zake pa kadyetsedwe ka carp ndi kupititsa patsogolo kukula, ndikupereka deta yokhudzana ndi kufalikira kwa zokopa ziwiri zatsopanozi m'mafakitale a zakudya ndi zamoyo zam'madzi.

1 Zipangizo ndi Njira

1.1 Zida zoyesera ndi nsomba zoyesera
S. S' - Dimethylacetic acid thiazole (Chithunzi cha DMT, DMT
Carp yoyesera idatengedwa kuchokera ku famu ya aquaculture, yokhala ndi matupi athanzi komanso mawonekedwe abwino. Kuyesera kusanayambe, nsomba zoyesera zidzakwezedwa kwakanthawi mu labotale kwa masiku 7, pomwe zimadyetsedwa ndi chakudya cha carp choperekedwa ndi fakitale yazakudya.
1.2 Zakudya zoyesera
1.2.1 Chakudya choyesera chokopa: Gwirani chakudya cha carp choperekedwa ndi fakitale ya chakudya, onjezerani kuchuluka kwa A-starch, sakanizani mofanana, ndi kusakaniza ndi madzi okwanira osungunuka kuti mupange mipira yomata 5g iliyonse monga gulu lolamulira. Pa nthawi yomweyo, konzani nyambo chakudya choyamba kuphwanya carp chakudya, kuwonjezera kuchuluka kwa alpha wowuma, ndi kuwonjezera nyambo DMT ndi.DMPTpazigawo ziwiri za 0.5g/kg ndi 1g/kg, motero. Sakanizani mofanana ndikusakaniza ndi madzi okwanira okwanira kuti mupange mpira womata 5g uliwonse.
1.2.2 Mayeso a Kukula:

Gwirani chakudya cha carp (kuchokera ku gwero lomwelo) kukhala ufa, perekani mu sieve ya 60 mesh, onjezerani kuchuluka kwa alpha starch, sakanizani bwino, sakanizani ndi madzi osungunuka, finyani kuchokera ku sieve kukhala ma granules, ndikuwumitsa mpweya kuti mupeze gulu lolamulira chakudya cha mayeso a kukula. The apangaChithunzi cha DMTndi makhiristo a DMPT adasungunuka m'madzi osungunuka kuti akonze njira yothetsera ndende yoyenera, yomwe idagwiritsidwa ntchito kusakaniza chakudya cha carp chosakanikirana bwino ndi wowuma mu granules. Pambuyo kuyanika, kuyesa gulu chakudya anapezedwa, ndiChithunzi cha DMTndi DMPT anawonjezera m'magulu atatu ndende ya 0.1g/kg, 0.2g/kg, ndi 0.3g/kg, motero.

1.3 Njira Yoyesera
1.3.1 Mayeso okopa: Sankhani carp 5 yoyesera (yolemera pafupifupi 30g) ngati nsomba yoyesera. Asanayambe kuyesedwa, idyani njala kwa maola 24, ndiyeno ikani nsomba zoyesedwa mu galasi lamadzimadzi (lokhala ndi kukula kwa 40 × 30 × 25cm). Chakudya cha nyambocho chimakhazikika pa mtunda wa 5.0cm kuchokera pansi pa aquarium pogwiritsa ntchito chingwe choyimitsidwa chomangirira pa bala yopingasa. Nsombazo zimaluma nyamboyo n’kunjenjemera chingwecho, chomwe chimatumizidwa ku kapamwamba kopingasa n’kujambulidwa ndi makina ojambulira magudumu. Kuchuluka kwa nyambo kumawerengeredwa potengera kugwedezeka kwakukulu kwa nsomba 5 zoyeserera zomwe zimaluma nyambo mkati mwa mphindi ziwiri. Mayeso odyetsera gulu lililonse la chakudya adabwerezedwa katatu, pogwiritsa ntchito mipira yomatira yomwe yakonzedwa kumene nthawi iliyonse. Pochita zoyeserera mobwerezabwereza kuti apeze chiwerengero chonse ndi pafupifupi pafupipafupi kunyambo, kudyetsa zotsatira zaChithunzi cha DMTndi DMPT pa carp ikhoza kuyesedwa.

1.3.2 Kuyesera kwa kukula kumagwiritsa ntchito magalasi a 8 amadzimadzi (kukula kwa 55 × 45 × 50cm), ndi madzi akuya a 40cm, kutentha kwamadzi achilengedwe, ndi kukwera kwa inflation kosalekeza. Nsomba zoyesera zinaperekedwa mwachisawawa ndikugawidwa m'magulu awiri kuti ayesedwe. Gulu loyamba lili ndi ma aquariums anayi, owerengeka X1 (gulu lolamulira), X2 (0.1gDMT/kg feed), X3 (0.2gDMT/kg feed), X4 (0.3gDMT/kg feed); Gulu lina la 4 aquariums, lowerengedwa Y1 (gulu lolamulira), Y2 (0.10g DMPT/kg feed), Y3 (0.2g DMPT/kg feed), Y4 (0.30g DMPT/kg feed). 20 nsomba pa bokosi, kudyetsedwa 3 pa tsiku pa 8:00, 13:00, ndi 17:00, ndi tsiku kudya mlingo wa 5-7% ya kulemera kwa thupi. Kuyeserako kunatenga masabata a 6. Kumayambiriro ndi kumapeto kwa kuyesera, kulemera konyowa kwa nsomba zoyesedwa kunayesedwa ndipo chiwerengero cha kupulumuka kwa gulu lirilonse chinalembedwa.

2.1 Kudyetsa kwa DMPT ndiChithunzi cha DMTku carp
Kudyetsa kwa DMPT ndiChithunzi cha DMTpa carp ikuwonetsedwa ndi kuluma kwafupipafupi kwa nsomba zoyesera panthawi ya kuyesera kwa mphindi ya 2, monga momwe tawonetsera mu Table 1. Kuyesera kunapeza kuti atatha kuwonjezera DMPT ndi DMT chakudya ku aquarium, nsomba yoyesera mwamsanga inasonyeza khalidwe logwira ntchito lodyera, pamene pogwiritsira ntchito gulu lolamulira chakudya, zomwe nsomba zoyeserazo zinkachita zinali pang'onopang'ono. Poyerekeza ndi chakudya chowongolera, nsomba zoyesera zinali ndi kuwonjezeka kwakukulu kwafupipafupi kuluma chakudya choyesera. DMT ndi DMPT ali ndi zotsatira zokopa kwambiri pa carp yoyesera.

Kulemera kwa kulemera, kukula kwapadera, ndi kupulumuka kwa carp kudyetsedwa ndi magulu osiyanasiyana a DMPT kunawonjezeka kwambiri poyerekeza ndi omwe amadyetsedwa ndi chakudya chowongolera, pamene chakudya chodyera chinachepetsedwa kwambiri. Pakati pawo, kuwonjezera kwa DMPT ku T2, T3, ndi T4 kunawonjezera kulemera kwa tsiku ndi tsiku kwa magulu atatu ndi 52.94%, 78.43%, ndi 113.73%, motero, poyerekeza ndi gulu lolamulira. Kulemera kwa chiwerengero cha T2, T3, ndi T4 chinawonjezeka ndi 60.44%, 73.85%, ndi 98.49%, motero, ndi kukula kwapadera kwawonjezeka ndi 41.22%, 51.15%, ndi 60.31%, motero. Ziwerengero zopulumuka zidakwera kuchoka pa 90% mpaka 95%, ndipo ma coefficients a chakudya adatsika ndi 28.01%, 29.41%, ndi 33.05%, motsatana.

3. Mapeto

Mu kuyesera uku, kayaChithunzi cha DMTkapena DMPT inawonjezeredwa, kudyetsa pafupipafupi, kukula kwapadera, ndi kulemera kwa tsiku ndi tsiku kwa nsomba zoyesera m'gulu lililonse zinawonjezeka kwambiri poyerekeza ndi gulu lolamulira, pamene chiwerengero cha chakudya chinachepa kwambiri. Ndipo kaya ndi DMT kapena DMPT, zotsatira zolimbikitsa kukula zimakhala zofunikira kwambiri ndi kuwonjezeka kwa ndalama zowonjezera muzinthu zitatu za 0.1g/kg, 0.2g/kg, ndi 0.3g/kg. Panthawi imodzimodziyo, kuyerekezera kwa kudyetsa ndi kukulitsa zotsatira zolimbikitsa za DMT ndi DMPT zinapangidwa. Zinapezeka kuti pansi pa kumeta tsitsi komweko, kudyetsa pafupipafupi, kulemera kwa kulemera, ndi kukula kwapadera kwa nsomba zoyesera mu gulu la chakudya cha DMPT zinawonjezeka kwambiri poyerekeza ndi gulu la chakudya cha DMT, pamene chiwerengero cha chakudya chinachepetsedwa kwambiri. Kunena zoona, DMPT imakhudza kwambiri kukopa ndi kulimbikitsa kukula kwa carp poyerekeza ndi DMT. Kuyesera kumeneku kunagwiritsa ntchito DMPT ndi DMT yowonjezeredwa ku chakudya cha carp kuti awone momwe amadyetsa komanso kukula kwawo. Zotsatira zikuwonetsa kuti DMPT ndi DMT ali ndi chiyembekezo chogwiritsa ntchito ngati m'badwo watsopano wokopa nyama zam'madzi.