Zokopa zamphamvu kwambiriDMPTndiDMTndi zinthu zatsopano komanso zothandiza kwambiri zokopa nyama zam'madzi. Mu kafukufukuyu, zinthu zokopa zamphamvu kwambiriDMPTndiDMTzinawonjezedwa ku chakudya cha carp kuti zifufuze zotsatira za zokopa ziwirizi pa kudyetsa carp ndi kukulitsa kukula. Zotsatira zake zinasonyeza kuti kuwonjezera kwa zokopa zamphamvu kwambiriDMPTndiDMTku chakudya kunawonjezera kwambiri kuchuluka kwa kuluma kwa nsomba zoyesera ndipo kunali ndi zotsatira zazikulu pakudyetsa; Nthawi yomweyo, kuwonjezera kuchuluka kosiyanasiyana kwa zokopa zamphamvu kwambiriDMPTndiDMTKupita ku chakudya kunawonjezera kwambiri kuchuluka kwa kulemera, kuchuluka kwa kukula, komanso kuchuluka kwa kupulumuka kwa nsomba zoyesera, pomwe kuchuluka kwa chakudya kunachepa kwambiri. Zotsatira za kafukufuku zikuwonetsanso kutiDMPTali ndi mphamvu yofunikira kwambiri pakukopa ndikulimbikitsa kukula kwa carp poyerekeza ndiDMT.

Chokoka chakudya cha ziweto m'madzi si chakudya chopatsa thanzi. Kuwonjezera zinthu zokoka kuti zidyetse nsomba kungathandize kwambiri kudyetsa nsomba, kuwonjezera chakudya chomwe zimadya, kuchepetsa chakudya chotsala m'madzi, motero kuchepetsa kuipitsa madzi m'madzi okhala ndi nsomba.DMPTndiDMTNdi zinthu zogwira ntchito zomwe zimapezeka kwambiri m'zamoyo zam'madzi, zomwe zimagwira ntchito ngati othandizira a methyl komanso olamulira ofunikira a osmotic pressure. Zimakhalanso ndi zotsatira zazikulu pakudyetsa ndi kukula kwa nyama zam'madzi.

Ofufuza aku Japan atachita kafukufuku wofunikira pa nyama zam'madzi monga crucian carp, red snapper, goldfish, ndi spotted shrimp, adapeza kutiDMPTndiDMTZimakopa nsomba za m'madzi oyera komanso zam'madzi, nkhanu, ndi nkhono. Zimawonjezera kuchuluka kochepa kwa zinthu zokopa zamphamvu kwambiri.DMPTndiDMTZakudya za nsomba za m'madzi amchere zimatha kufulumizitsa kwambiri kudyetsa ndi kukula kwa nsomba zosiyanasiyana za m'madzi amchere ndi zam'madzi. Mu kuyesera uku, nsomba zokopa kwambiriDMPTndiDMTZawonjezeredwa ku chakudya cha carp kuti ziphunzire momwe zimakhudzira kudyetsa carp ndi kukulitsa kukula, zomwe zimapereka deta yofotokozera momwe zinthu ziwiri zatsopanozi zimagwiritsidwira ntchito kwambiri m'mafakitale odyetsera chakudya ndi ulimi wa nsomba.

1 Zipangizo ndi Njira

1.1 Zipangizo zoyesera ndi nsomba zoyesera
S. S' - Dimethylacetic acid thiazole (DMT), DMPT
Nsomba zoyeserera za carp zinatengedwa kuchokera ku famu yoweta nsomba, yokhala ndi matupi athanzi komanso zofunikira bwino. Kuyesaku kusanayambe mwalamulo, nsomba zoyesererazi zidzaleredwa kwakanthawi mu labotale kwa masiku 7, pomwe zidzadyetsedwa ndi chakudya cha carp choperekedwa ndi fakitale yodyetsa.
1.2 Kudyetsa koyesera
1.2.1 Chakudya choyesera nyambo: Pukutani chakudya cha carp chomwe chimaperekedwa ndi fakitale yodyetsa, onjezerani kuchuluka kofanana kwa sitachi ya A, sakanizani mofanana, ndikusakaniza ndi madzi okwanira kuti mupange mipira yomata ya 5g iliyonse ngati chakudya cha gulu lolamulira. Nthawi yomweyo, konzani chakudya cha nyambo poyamba kuphwanya chakudya cha carp, kuwonjezera kuchuluka kofanana kwa sitachi ya alpha, ndikuwonjezera DMT ndi nyamboDMPTPawiri pa 0.5g/kg ndi 1g/kg, motsatana. Sakanizani mofanana ndikusakaniza ndi madzi osungunuka okwanira kuti mupange mpira uliwonse wa 5g womata.
1.2.2 Chakudya choyesera kukula:

Dulani chakudya cha carp (chochokera ku gwero lomweli monga pamwambapa) kukhala ufa, chidutseni mu sefa ya maukonde 60, onjezerani alpha starch yofanana, sakanizani bwino, sakanizani ndi madzi osungunuka, finyani kuchokera mu sefa kukhala tinthu tating'onoting'ono, ndikuwumitsa ndi mpweya kuti mupeze chakudya cha gulu lolamulira kuti muyese kukula.DMTndipo makhiristo a DMPT anasungunuka m'madzi osungunuka kuti akonze yankho loyenera, lomwe linagwiritsidwa ntchito kusakaniza chakudya cha carp ndi starch chosakanikirana bwino kukhala tinthu tating'onoting'ono. Pambuyo pouma, chakudya cha gulu loyesera chinapezeka, ndiDMTndipo DMPT inawonjezeredwa m'magawo atatu a 0.1g/kg, 0.2g/kg, ndi 0.3g/kg, motsatana.

1.3 Njira Yoyesera
1.3.1 Mayeso a nyambo: Sankhani nsomba 5 zoyeserera za carp (zolemera pafupifupi 30g) ngati nsomba yoyesera. Musanayese, idyani chakudya kwa maola 24, kenako ikani nsomba yoyeserayo mu aquarium yagalasi (yokhala ndi kukula kwa 40 × 30 × 25cm). Chakudya cha nyambo chimakhazikika pa mtunda wa 5.0cm kuchokera pansi pa aquarium pogwiritsa ntchito chingwe chopachikidwa chomangiriridwa ku bar yopingasa. Nsomba imaluma nyambo ndikugwedezeka mzerewo, womwe umatumizidwa ku bar yopingasa ndikujambulidwa ndi chojambulira mawilo. Kuchuluka kwa kuluma kwa nyambo kumawerengedwa kutengera kugwedezeka kwakukulu kwa nsomba 5 zoyeserera zomwe zimaluma nyambo mkati mwa mphindi ziwiri. Mayeso odyetsa gulu lililonse la chakudya adabwerezedwa katatu, pogwiritsa ntchito mipira yomatira yodyetsedwa yatsopano nthawi iliyonse. Pochita mayeso obwerezabwereza kuti mupeze chiwerengero chonse ndi kuchuluka kwa nyambo, zotsatira za kudyetsa zaDMTndipo DMPT pa carp ikhoza kuyesedwa.

1.3.2 Kuyesera kukula kumagwiritsa ntchito malo osungiramo madzi agalasi 8 (kukula kwa 55 × 45 × 50cm), okhala ndi kuya kwa madzi kwa 40cm, kutentha kwachilengedwe kwa madzi, komanso kukwera kwa madzi kosalekeza. Nsomba zoyesera zinagawidwa mwachisawawa ndipo zinagawidwa m'magulu awiri pa kuyesaku. Gulu loyamba lili ndi malo osungiramo madzi anayi, omwe ali ndi manambala X1 (gulu lowongolera), X2 (0.1gDMT/kg feed), X3 (0.2gDMT/kg feed), X4 (0.3gDMT/kg feed); Gulu lina la malo osungiramo madzi anayi, omwe ali ndi manambala Y1 (gulu lowongolera), Y2 (0.10g DMPT/kg feed), Y3 (0.2g DMPT/kg feed), Y4 (0.30g DMPT/kg feed). Nsomba 20 pa bokosi lililonse, zimadyetsedwa katatu patsiku nthawi ya 8:00, 13:00, ndi 17:00, ndi kuchuluka kwa chakudya cha tsiku ndi tsiku cha 5-7% ya kulemera kwa thupi. Kuyeseraku kunatenga milungu 6. Poyamba ndi kumapeto kwa kuyesera, kulemera kwa nsomba yoyesera kunayesedwa ndipo kuchuluka kwa kupulumuka kwa gulu lililonse kunalembedwa.

2.1 Mphamvu yodyetsera ya DMPT ndiDMTpa kapu
Zotsatira za DMPT ndi kudyaDMTKuchuluka kwa nsomba pa carp kumaonekera chifukwa cha kuchuluka kwa kuluma kwa nsomba zoyesera panthawi yoyesera ya mphindi ziwiri, monga momwe zasonyezedwera mu Gome 1. Kuyeseraku kunapeza kuti pambuyo powonjezera chakudya cha DMPT ndi DMT ku aquarium, nsomba zoyesera zinawonetsa mwachangu momwe zimafunira chakudya, pomwe zikagwiritsa ntchito chakudya cha gulu lowongolera, momwe nsomba zoyesera zimachitira zinali zochepa. Poyerekeza ndi chakudya chowongolera, nsomba zoyesera zinali ndi kuwonjezeka kwakukulu kwa kuchuluka kwa kuluma chakudya choyesera. DMT ndi DMPT zimakhala ndi zotsatira zokopa kwambiri pa carp yoyesera.

Kuchuluka kwa kulemera, kuchuluka kwa kukula, ndi kuchuluka kwa moyo wa carp wodyetsedwa ndi kuchuluka kosiyanasiyana kwa DMPT kunawonjezeka kwambiri poyerekeza ndi omwe amadyetsedwa ndi chakudya chowongolera, pomwe kuchuluka kwa chakudya kunachepetsedwa kwambiri. Pakati pawo, kuwonjezera kwa DMPT ku T2, T3, ndi T4 kunawonjezera kulemera kwa tsiku ndi tsiku kwa magulu atatuwa ndi 52.94%, 78.43%, ndi 113.73%, motsatana, poyerekeza ndi gulu lowongolera. Kuchuluka kwa kulemera kwa T2, T3, ndi T4 kunawonjezeka ndi 60.44%, 73.85%, ndi 98.49%, motsatana, ndipo kuchuluka kwa kukula kunawonjezeka ndi 41.22%, 51.15%, ndi 60.31%, motsatana. Kuchuluka kwa moyo kunakwera kuchoka pa 90% mpaka 95%, ndipo kuchuluka kwa chakudya kunachepa ndi 28.01%, 29.41%, ndi 33.05%, motsatana.

3. Mapeto

Mu kuyesera uku, kayaDMTkapena DMPT itawonjezedwa, kuchuluka kwa chakudya, kuchuluka kwa kukula, ndi kulemera kwa tsiku ndi tsiku kwa nsomba zoyesera mu gulu lililonse zidawonjezeka kwambiri poyerekeza ndi gulu lolamulira, pomwe kuchuluka kwa chakudya kudatsika kwambiri. Ndipo kaya ndi DMT kapena DMPT, mphamvu yolimbikitsira kukula imakhala yofunika kwambiri ndi kuwonjezeka kwa kuchuluka kowonjezera m'magulu atatu a 0.1g/kg, 0.2g/kg, ndi 0.3g/kg. Nthawi yomweyo, kufananiza kwa zotsatira zolimbikitsa kudya ndi kukula kwa DMT ndi DMPT kudapangidwa. Zinapezeka kuti pansi pa kuchuluka komweko kwa tsitsi, kuchuluka kwa chakudya, kuchuluka kwa kulemera, ndi kuchuluka kwa kukula kwa nsomba zoyeserera mu gulu la chakudya cha DMPT zidawonjezeka kwambiri poyerekeza ndi gulu la chakudya cha DMT, pomwe kuchuluka kwa chakudya kudachepetsedwa kwambiri. Ponena za izi, DMPT ili ndi zotsatira zazikulu pakukopa ndikulimbikitsa kukula kwa carp poyerekeza ndi DMT. Kuyesera kumeneku kunagwiritsa ntchito DMPT ndi DMT zomwe zidawonjezeredwa ku chakudya cha carp kuti zifufuze zotsatira zawo zolimbikitsa kudya ndi kukula. Zotsatira zake zikuwonetsa kuti DMPT ndi DMT zili ndi mwayi waukulu wogwiritsidwa ntchito ngati mbadwo watsopano wa zokopa nyama zam'madzi.